compounds 3a 3e against methicillin Search Results


3e 1 56 3a pseudomonas syringae pv  (ATCC)
97
ATCC 3e 1 56 3a pseudomonas syringae pv
3e 1 56 3a Pseudomonas Syringae Pv, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t5 caption a7 yeast strains kanb 3a 3b 3c 3d 3e 4c 4d pos itc flc c albicans atcc 10231  (ATCC)
97
ATCC t5 caption a7 yeast strains kanb 3a 3b 3c 3d 3e 4c 4d pos itc flc c albicans atcc 10231
MIC Values ( μ g/mL) a of KANA, <t> KANB, </t> 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains
T5 Caption A7 Yeast Strains Kanb 3a 3b 3c 3d 3e 4c 4d Pos Itc Flc C Albicans Atcc 10231, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t5 caption a7 yeast strains kanb 3a 3b 3c 3d 3e 4c 4d pos itc flc c albicans atcc 10231 - by Bioz Stars, 2026-11
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catalog number 1a alcohol  (Chem Impex International)
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Chem Impex International catalog number 1a alcohol
MIC Values ( μ g/mL) a of KANA, <t> KANB, </t> 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains
Catalog Number 1a Alcohol, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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orca-flash 4 camera  (Hamamatsu)
90
Hamamatsu orca-flash 4 camera
MIC Values ( μ g/mL) a of KANA, <t> KANB, </t> 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains
Orca Flash 4 Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human semaphorin 3e sema3e elisa  (Cusabio)
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Cusabio human semaphorin 3e sema3e elisa
MIC Values ( μ g/mL) a of KANA, <t> KANB, </t> 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains
Human Semaphorin 3e Sema3e Elisa, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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coordinate files for 6α-crellasterone a (2a; minus sidechain)  (TURBOMOLE GmbH)
90
TURBOMOLE GmbH coordinate files for 6α-crellasterone a (2a; minus sidechain)
MIC Values ( μ g/mL) a of KANA, <t> KANB, </t> 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains
Coordinate Files For 6α Crellasterone A (2a; Minus Sidechain), supplied by TURBOMOLE GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti p63 primary antibody  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mouse monoclonal anti p63 primary antibody
Fig. 3. Fabrication of oral mucosal epithelial cell sheets. A. Morphology of cultured oral mucosal epithelial cells at day 7. Bar = 50 μm. B. Macroscopic observation of the oral mucosal epithelial cell sheet. The epithelial cell sheet was of sufficient size for transplantation over the wound in the ear canal of the rabbit. Bar = 20 mm. C. Colony-forming assay (CFA) of oral mucosal epithelial cells. The cells were seeded at a density of 1 × 10 5 cells/cm 2 and co-cultured with NIH-3T3 cells treated with mitomycin C. The colony-forming efficiency was 0.7%. D. CFA of epithelial cells derived from the cell sheet. The colony-forming efficiency was 1.0%. E. Histological observations of the cell sheets. HE: hematoxylin-eosin; pCK: pancytokeratin. The cells comprising the sheets were positive for pancytokeratin and <t>p63,</t> and the cells formed stratified layers.
Mouse Monoclonal Anti P63 Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek 293t  (Verlag GmbH)
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Verlag GmbH hek 293t
Fig. 3. Fabrication of oral mucosal epithelial cell sheets. A. Morphology of cultured oral mucosal epithelial cells at day 7. Bar = 50 μm. B. Macroscopic observation of the oral mucosal epithelial cell sheet. The epithelial cell sheet was of sufficient size for transplantation over the wound in the ear canal of the rabbit. Bar = 20 mm. C. Colony-forming assay (CFA) of oral mucosal epithelial cells. The cells were seeded at a density of 1 × 10 5 cells/cm 2 and co-cultured with NIH-3T3 cells treated with mitomycin C. The colony-forming efficiency was 0.7%. D. CFA of epithelial cells derived from the cell sheet. The colony-forming efficiency was 1.0%. E. Histological observations of the cell sheets. HE: hematoxylin-eosin; pCK: pancytokeratin. The cells comprising the sheets were positive for pancytokeratin and <t>p63,</t> and the cells formed stratified layers.
Hek 293t, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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methoxyphenyl 6 tosyl 6h imidazo 1 5 a pyrrolo 2 3 e pyrazin  (Chem Impex International)
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Chem Impex International methoxyphenyl 6 tosyl 6h imidazo 1 5 a pyrrolo 2 3 e pyrazin
Fig. 3. Fabrication of oral mucosal epithelial cell sheets. A. Morphology of cultured oral mucosal epithelial cells at day 7. Bar = 50 μm. B. Macroscopic observation of the oral mucosal epithelial cell sheet. The epithelial cell sheet was of sufficient size for transplantation over the wound in the ear canal of the rabbit. Bar = 20 mm. C. Colony-forming assay (CFA) of oral mucosal epithelial cells. The cells were seeded at a density of 1 × 10 5 cells/cm 2 and co-cultured with NIH-3T3 cells treated with mitomycin C. The colony-forming efficiency was 0.7%. D. CFA of epithelial cells derived from the cell sheet. The colony-forming efficiency was 1.0%. E. Histological observations of the cell sheets. HE: hematoxylin-eosin; pCK: pancytokeratin. The cells comprising the sheets were positive for pancytokeratin and <t>p63,</t> and the cells formed stratified layers.
Methoxyphenyl 6 Tosyl 6h Imidazo 1 5 A Pyrrolo 2 3 E Pyrazin, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4a 12  (Bruker Corporation)
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Bruker Corporation 4a 12
Fig. 3. Fabrication of oral mucosal epithelial cell sheets. A. Morphology of cultured oral mucosal epithelial cells at day 7. Bar = 50 μm. B. Macroscopic observation of the oral mucosal epithelial cell sheet. The epithelial cell sheet was of sufficient size for transplantation over the wound in the ear canal of the rabbit. Bar = 20 mm. C. Colony-forming assay (CFA) of oral mucosal epithelial cells. The cells were seeded at a density of 1 × 10 5 cells/cm 2 and co-cultured with NIH-3T3 cells treated with mitomycin C. The colony-forming efficiency was 0.7%. D. CFA of epithelial cells derived from the cell sheet. The colony-forming efficiency was 1.0%. E. Histological observations of the cell sheets. HE: hematoxylin-eosin; pCK: pancytokeratin. The cells comprising the sheets were positive for pancytokeratin and <t>p63,</t> and the cells formed stratified layers.
4a 12, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vibratory sieve shaker  (FRITSCH GmbH)
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FRITSCH GmbH vibratory sieve shaker
Fig. 3. Fabrication of oral mucosal epithelial cell sheets. A. Morphology of cultured oral mucosal epithelial cells at day 7. Bar = 50 μm. B. Macroscopic observation of the oral mucosal epithelial cell sheet. The epithelial cell sheet was of sufficient size for transplantation over the wound in the ear canal of the rabbit. Bar = 20 mm. C. Colony-forming assay (CFA) of oral mucosal epithelial cells. The cells were seeded at a density of 1 × 10 5 cells/cm 2 and co-cultured with NIH-3T3 cells treated with mitomycin C. The colony-forming efficiency was 0.7%. D. CFA of epithelial cells derived from the cell sheet. The colony-forming efficiency was 1.0%. E. Histological observations of the cell sheets. HE: hematoxylin-eosin; pCK: pancytokeratin. The cells comprising the sheets were positive for pancytokeratin and <t>p63,</t> and the cells formed stratified layers.
Vibratory Sieve Shaker, supplied by FRITSCH GmbH, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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forskolin  (Chem Impex International)
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Chem Impex International forskolin
Fig. 3. Fabrication of oral mucosal epithelial cell sheets. A. Morphology of cultured oral mucosal epithelial cells at day 7. Bar = 50 μm. B. Macroscopic observation of the oral mucosal epithelial cell sheet. The epithelial cell sheet was of sufficient size for transplantation over the wound in the ear canal of the rabbit. Bar = 20 mm. C. Colony-forming assay (CFA) of oral mucosal epithelial cells. The cells were seeded at a density of 1 × 10 5 cells/cm 2 and co-cultured with NIH-3T3 cells treated with mitomycin C. The colony-forming efficiency was 0.7%. D. CFA of epithelial cells derived from the cell sheet. The colony-forming efficiency was 1.0%. E. Histological observations of the cell sheets. HE: hematoxylin-eosin; pCK: pancytokeratin. The cells comprising the sheets were positive for pancytokeratin and <t>p63,</t> and the cells formed stratified layers.
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Image Search Results


MIC Values ( μ g/mL) a of KANA, KANB, 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: MIC Values ( μ g/mL) a of KANA, KANB, 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques:

Bar graph displaying the relative initial rates of reactions of the various AMEs with KANB and its derivatives 3a–e and 4c, d. Rates are normalized to KANB.

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: Bar graph displaying the relative initial rates of reactions of the various AMEs with KANB and its derivatives 3a–e and 4c, d. Rates are normalized to KANB.

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques:

MIC Values ( μ g/mL) Determined for KANB, Its Derivatives 3a–e and 4c, d, and Three Control Antifungal Agents (POS, ITC, and FLC) against Various Yeast Strains and Filamentous Fungi a

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: MIC Values ( μ g/mL) Determined for KANB, Its Derivatives 3a–e and 4c, d, and Three Control Antifungal Agents (POS, ITC, and FLC) against Various Yeast Strains and Filamentous Fungi a

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques: Control

Representative time-kill studies of KANB derivatives 3c and 3d against azole-resistant C. albicans ATCC 64124 (strain B). (A) Cultures were exposed to 3c at 8 μg/mL (○), 16 μg/mL (▼), and 32 μg/mL (△). (B) Cultures were exposed to 3d at 2 μg/mL (○), 4 μg/mL (▼), and 8 μg/mL (△). In both panels, cultures were exposed to AmB at 1 μg/mL (■) or to a no drug control (●).

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: Representative time-kill studies of KANB derivatives 3c and 3d against azole-resistant C. albicans ATCC 64124 (strain B). (A) Cultures were exposed to 3c at 8 μg/mL (○), 16 μg/mL (▼), and 32 μg/mL (△). (B) Cultures were exposed to 3d at 2 μg/mL (○), 4 μg/mL (▼), and 8 μg/mL (△). In both panels, cultures were exposed to AmB at 1 μg/mL (■) or to a no drug control (●).

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques: Control

(A) Representative dose-dependent membrane permeabilization effects of KANB and its derivatives 3c and 3d on azole-resistant C. albicans ATCC 64124 (B). From top to bottom: Propidium iodine (PI) dye uptake by yeast cells without drug, with KANB (62.5 μg/mL), with 3c (1× and 2× MIC), and with 3d (1× and 2× MIC). (B) Quantitative representation of the images shown in panel A.

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: (A) Representative dose-dependent membrane permeabilization effects of KANB and its derivatives 3c and 3d on azole-resistant C. albicans ATCC 64124 (B). From top to bottom: Propidium iodine (PI) dye uptake by yeast cells without drug, with KANB (62.5 μg/mL), with 3c (1× and 2× MIC), and with 3d (1× and 2× MIC). (B) Quantitative representation of the images shown in panel A.

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques: Membrane

Hemolytic activity of KANB, gramicidin, amphotericin B (AmB), and 3a–e on mouse red blood cells.

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: Hemolytic activity of KANB, gramicidin, amphotericin B (AmB), and 3a–e on mouse red blood cells.

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques: Activity Assay

Mammalian cell cytotoxicity of KANB and its derivatives 3a–d against (A) A549 cell line and (B) BEAS-2B cell line.

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: Mammalian cell cytotoxicity of KANB and its derivatives 3a–d against (A) A549 cell line and (B) BEAS-2B cell line.

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques:

Fig. 3. Fabrication of oral mucosal epithelial cell sheets. A. Morphology of cultured oral mucosal epithelial cells at day 7. Bar = 50 μm. B. Macroscopic observation of the oral mucosal epithelial cell sheet. The epithelial cell sheet was of sufficient size for transplantation over the wound in the ear canal of the rabbit. Bar = 20 mm. C. Colony-forming assay (CFA) of oral mucosal epithelial cells. The cells were seeded at a density of 1 × 10 5 cells/cm 2 and co-cultured with NIH-3T3 cells treated with mitomycin C. The colony-forming efficiency was 0.7%. D. CFA of epithelial cells derived from the cell sheet. The colony-forming efficiency was 1.0%. E. Histological observations of the cell sheets. HE: hematoxylin-eosin; pCK: pancytokeratin. The cells comprising the sheets were positive for pancytokeratin and p63, and the cells formed stratified layers.

Journal: Acta biomaterialia

Article Title: Transplantation of autologous oral mucosal epithelial cell sheets inhibits the development of acquired external auditory canal atresia in a rabbit model.

doi: 10.1016/j.actbio.2020.04.031

Figure Lengend Snippet: Fig. 3. Fabrication of oral mucosal epithelial cell sheets. A. Morphology of cultured oral mucosal epithelial cells at day 7. Bar = 50 μm. B. Macroscopic observation of the oral mucosal epithelial cell sheet. The epithelial cell sheet was of sufficient size for transplantation over the wound in the ear canal of the rabbit. Bar = 20 mm. C. Colony-forming assay (CFA) of oral mucosal epithelial cells. The cells were seeded at a density of 1 × 10 5 cells/cm 2 and co-cultured with NIH-3T3 cells treated with mitomycin C. The colony-forming efficiency was 0.7%. D. CFA of epithelial cells derived from the cell sheet. The colony-forming efficiency was 1.0%. E. Histological observations of the cell sheets. HE: hematoxylin-eosin; pCK: pancytokeratin. The cells comprising the sheets were positive for pancytokeratin and p63, and the cells formed stratified layers.

Article Snippet: The sections were then ncubated with a solution to block endogenous peroxidase activty (S2023; Dako) at 20–30 °C for 30 min and with a blocking eagent (to block nonspecific reactions) at 20–30 °C for 1 h. The ections were treated with mouse monoclonal anti-pancytokeratin AE1/AE3) primary antibody (1:100 dilution; Abcam, Cambridge, K; catalog no. ab27988), mouse monoclonal anti-vimentin (clone 9) primary antibody (1:100 dilution; Dako; catalog no. GA63061- J) or mouse monoclonal anti-p63 primary antibody (1:100 diution; Santa Cruz Biotechnology, Santa Cruz, CA, USA; catalog n w V 2 ( 2 H o t t 3 3 a e T a d a d d o c a h 3 e o. sc-8431) at 4 °C overnight.

Techniques: Cell Culture, Transplantation Assay, Derivative Assay